Buying an Automotive Scope would be the best way forward, the Automotive Scopes have thermal protection fuses to help protect the scope in case you were to overload it. Plus the other main advantage is that the Automotive Scopes have a 100V input range where as the 4227 (being a test and measurement scope) only has 20V input, so injectors which can easily be looked at with the 4000 series Automotive Scope would need attenuating with the 4227 so you dont damage it.
The 4227 will not work in the Automotive Software as its not desinged or constructed for this purpose so it wont be validated for use by the software. Only the Automotive Scopes will function in the automotive software.
You can run 2 scopes at the same time i.e. 1 in 1 version of software and 2 in a second version of the software but there will be a time difference so when triggering in 1 instance of software and triggering in another the triggers wont be in line. This will therefore give you an offset in the readings which really is not the best way to do this especially when looking at responses from various inputs such as sensors.
I would really recommend getting the 4423 scope as so many users who buy the 4223 scope alwasy come back for a 4 channel scope as 2 channels on modern engines just isnt enough.
I hope this helps to answer your question
Lee - Automotive Technical Specialist